Mouse GREM2/Prdc ELISA Kit PicoKine®

GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 99 95 96 96 1.63 1.6% Sample 2 279 281 251 268 269 11.9 4.4% Sample 3 843 895 830 812 845 30.89 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 99 95 96 96 1.63 1.6% Sample 1 95 99 95 96 96 1.63 1.6% Sample 2 279 281 251 268 269 11.9 4.4% Sample 2 279 281 251 268 269 11.9 4.4% Sample 3 843 895 830 812 845 30.89 3.6% Sample 3 843 895 830 812 845 30.89 3.6%

Highly expressed in the ovary, followed by brain, spleen, colon, kidney and uterus. In ovary expressed in granulosa cells of selective early antral follicles. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 279 843 99 268 803 Standard deviation 4.9% 13.67 34.56 6.03 16.1%2 35.33 CV (%) 4.1%% 4.9% 4.1% 6.1% 6.8% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 279 843 99 268 803 Standard deviation 4.9% 13.67 34.56 6.03 16.1%2 35.33 CV (%) 4.1%% 4.9% 4.1% 6.1% 6.8% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 279 843 99 268 803 Mean (pg/ml) 99 279 843 99 268 803 Standard deviation 4.9% 13.67 34.56 6.03 16.1%2 35.33 Standard deviation 4.9% 13.67 34.56 6.03 16.1%2 35.33 CV (%) 4.1%% 4.9% 4.1% 6.1% 6.8% 4.4% CV (%) 4.1%% 4.9% 4.1% 6.1% 6.8% 4.4%

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.093 0.156 0.273 0.493 0.895 1.656 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.093 0.156 0.273 0.493 0.895 1.656 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.093 0.156 0.273 0.493 0.895 1.656 2.725 O.D. 0.02 0.093 0.156 0.273 0.493 0.895 1.656 2.725

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.