Human GREM2/Prdc ELISA Kit PicoKine®

GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 139 149 126 139 8.33 5.9% Sample 2 522 566 575 538 550 21.26 3.8% Sample 3 1488 1697 1588 1757 1632 103.1 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 139 149 126 139 8.33 5.9% Sample 1 142 139 149 126 139 8.33 5.9% Sample 2 522 566 575 538 550 21.26 3.8% Sample 2 522 566 575 538 550 21.26 3.8% Sample 3 1488 1697 1588 1757 1632 103.1 6.3% Sample 3 1488 1697 1588 1757 1632 103.1 6.3%

Gremlin-2; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; Protein related to DAN and cerberus; GREM2; CKTSF1B2, DAND3, PRDC

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 522 1488 140 556 1359 Standard deviation 10.08 39.15 113.08 12.32 45.03 107.36 CV (%) 7.1% 7.5% 7.6% 8.8% 8.1% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 522 1488 140 556 1359 Standard deviation 10.08 39.15 113.08 12.32 45.03 107.36 CV (%) 7.1% 7.5% 7.6% 8.8% 8.1% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 522 1488 140 556 1359 Mean (pg/ml) 142 522 1488 140 556 1359 Standard deviation 10.08 39.15 113.08 12.32 45.03 107.36 Standard deviation 10.08 39.15 113.08 12.32 45.03 107.36 CV (%) 7.1% 7.5% 7.6% 8.8% 8.1% 7.9% CV (%) 7.1% 7.5% 7.6% 8.8% 8.1% 7.9%

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.094 0.156 0.267 0.472 0.846 1.43 2.103 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.094 0.156 0.267 0.472 0.846 1.43 2.103 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.094 0.156 0.267 0.472 0.846 1.43 2.103 O.D. 0.041 0.094 0.156 0.267 0.472 0.846 1.43 2.103

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.