Human Pan Trypsin ELISA Kit PicoKine®

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 100 85 91 90 6.13 6.8% Sample 2 261 265 235 246 251 11.98 4.7% Sample 3 887 951 881 978 924 41.42 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 100 85 91 90 6.13 6.8% Sample 1 85 100 85 91 90 6.13 6.8% Sample 2 261 265 235 246 251 11.98 4.7% Sample 2 261 265 235 246 251 11.98 4.7% Sample 3 887 951 881 978 924 41.42 4.4% Sample 3 887 951 881 978 924 41.42 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 261 887 82 235 820 Standard deviation 6.03 20.09 66.52 6.88 22.32 64.78 CV (%) 7.1% 7.7% 7.5% 8.4% 9.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 261 887 82 235 820 Standard deviation 6.03 20.09 66.52 6.88 22.32 64.78 CV (%) 7.1% 7.7% 7.5% 8.4% 9.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 261 887 82 235 820 Mean (pg/ml) 85 261 887 82 235 820 Standard deviation 6.03 20.09 66.52 6.88 22.32 64.78 Standard deviation 6.03 20.09 66.52 6.88 22.32 64.78 CV (%) 7.1% 7.7% 7.5% 8.4% 9.5% 7.9% CV (%) 7.1% 7.7% 7.5% 8.4% 9.5% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.047 0.117 0.178 0.287 0.429 0.724 1.255 2.087 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.047 0.117 0.178 0.287 0.429 0.724 1.255 2.087 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.047 0.117 0.178 0.287 0.429 0.724 1.255 2.087 O.D. 0.047 0.117 0.178 0.287 0.429 0.724 1.255 2.087

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.