Human ICAM5 ELISA Kit PicoKine®

Human ICAM5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ICAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Intercellular adhesion molecule 5 is a protein that in humans is encoded by the ICAM5 gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 274 247 258 248 256 10.84 4.2% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 3 4856 4750 4812 4563 4745 111.75 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 274 247 258 248 256 10.84 4.2% Sample 1 274 247 258 248 256 10.84 4.2% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 3 4856 4750 4812 4563 4745 111.75 2.3% Sample 3 4856 4750 4812 4563 4745 111.75 2.3%

Synonyms

Intercellular adhesion molecule 5; ICAM-5; Telencephalin; ICAM5; TLCN, TLN

Gene Name

ICAM5

Gene ID

7087

UniProt

Q9UMF0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A28-E570

Tissue Specificity

Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon.

Applications

ELISA

Field of Research

Cell Adhesion Proteins, Cell Type Markers, Membrane Proteins, Neuroscience, Tags & Cell Markers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9%

Sensitivity

<15 pg/mL

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

97116 MW

Specificity

Natural and recombinant human ICAM5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Intercellular adhesion molecule 5

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Intercellular adhesion molecule 5

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.