Human CD48/Slamf2 ELISA Kit PicoKine®

CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 100 101 104 104 5.12 4.9% Sample 2 615 601 670 606 623 27.59 4.4% Sample 3 2155 1979 1842 1829 1951 131.49 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 100 101 104 104 5.12 4.9% Sample 1 113 100 101 104 104 5.12 4.9% Sample 2 615 601 670 606 623 27.59 4.4% Sample 2 615 601 670 606 623 27.59 4.4% Sample 3 2155 1979 1842 1829 1951 131.49 6.7% Sample 3 2155 1979 1842 1829 1951 131.49 6.7%

CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; TCT.1; CD48; CD48; BCM1, BLAST1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 615 2155 117 667 2219 Standard deviation 5.53 46.12 129.3 6.66 58.69 157.54 CV (%) 4.9% 7.5% 6% 5.7% 8.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 615 2155 117 667 2219 Standard deviation 5.53 46.12 129.3 6.66 58.69 157.54 CV (%) 4.9% 7.5% 6% 5.7% 8.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 113 615 2155 117 667 2219 Mean (pg/ml) 113 615 2155 117 667 2219 Standard deviation 5.53 46.12 129.3 6.66 58.69 157.54 Standard deviation 5.53 46.12 129.3 6.66 58.69 157.54 CV (%) 4.9% 7.5% 6% 5.7% 8.8% 7.1% CV (%) 4.9% 7.5% 6% 5.7% 8.8% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.062 0.139 0.21 0.346 0.566 0.969 1.659 2.063 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.062 0.139 0.21 0.346 0.566 0.969 1.659 2.063 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.062 0.139 0.21 0.346 0.566 0.969 1.659 2.063 O.D. 0.062 0.139 0.21 0.346 0.566 0.969 1.659 2.063

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.