Mouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Mouse WIF1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF) -like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 40 45 38 40 2.58 6.4% Sample 2 298 281 269 282 282 10.3 3.6% Sample 3 975 1067 1053 1054 1037 36.36 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 40 45 38 40 2.58 6.4% Sample 1 40 40 45 38 40 2.58 6.4% Sample 2 298 281 269 282 282 10.3 3.6% Sample 2 298 281 269 282 282 10.3 3.6% Sample 3 975 1067 1053 1054 1037 36.36 3.5% Sample 3 975 1067 1053 1054 1037 36.36 3.5%

Synonyms

Wnt inhibitory factor 1; WIF-1; Wif1

Gene Name

Wif1

Gene ID

24117

UniProt

Q9WUA1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G29-W379

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expression highest in heart and lung. Lower in brain and eye.

Applications

ELISA

Field of Research

Secreted, Signaling Pathways, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

23.4 pg/mL - 1500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 298 975 37 309 914 Standard deviation 1.92 22.94 39 1.88 28.73 43.87 CV (%) 4.8% 7.7% 4% 4.8% 9.3% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 298 975 37 309 914 Standard deviation 1.92 22.94 39 1.88 28.73 43.87 CV (%) 4.8% 7.7% 4% 4.8% 9.3% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 298 975 37 309 914 Mean (pg/ml) 40 298 975 37 309 914 Standard deviation 1.92 22.94 39 1.88 28.73 43.87 Standard deviation 1.92 22.94 39 1.88 28.73 43.87 CV (%) 4.8% 7.7% 4% 4.8% 9.3% 4.8% CV (%) 4.8% 7.7% 4% 4.8% 9.3% 4.8%

Sensitivity

<10 pg/mL

Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

41590 MW

Specificity

Natural and recombinant mouse WIF1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Wnt inhibitory factor 1

Subcellular Location

Secreted.

Protein Name

Wnt inhibitory factor 1

Sample Data

Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.05 0.123 0.2 0.304 0.606 1.029 1.732 2.436 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.05 0.123 0.2 0.304 0.606 1.029 1.732 2.436 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.05 0.123 0.2 0.304 0.606 1.029 1.732 2.436 O.D. 0.05 0.123 0.2 0.304 0.606 1.029 1.732 2.436

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.