Mouse TREML1/Tlt 1 ELISA Kit PicoKine®

Mouse TREML1/Tlt 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Treml1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 101 73 80 88 4.92 5.6% Sample 2 274 355 327 330 322 23.79 7.4% Sample 3 1220 1892 1272 1457 1460 34.90 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 101 73 80 88 4.92 5.6% Sample 1 99 101 73 80 88 4.92 5.6% Sample 2 274 355 327 330 322 23.79 7.4% Sample 2 274 355 327 330 322 23.79 7.4% Sample 3 1220 1892 1272 1457 1460 34.90 2.4% Sample 3 1220 1892 1272 1457 1460 34.90 2.4%

Synonyms

Trem-like transcript 1 protein; TLT-1; Triggering receptor expressed on myeloid cells-like protein 1; Treml1; Tlt1

Gene Name

Treml1

Gene ID

71326

UniProt

Q8K558

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D21-P175

Tissue Specificity

Highly expressed in bone marrow leukocytes, splenic megakaryocytes and platelets. Detected in brain, liver and in peritoneal monocytes. .

Applications

ELISA

Field of Research

Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Platelets, Protein Phosphorylation, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL -4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 274 1220 84 285 1472 Standard deviation 5.64 21.37 40.99 4.44 16.25 72.72 CV (%) 5.7% 7.8% 3.4% 5.3% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 274 1220 84 285 1472 Standard deviation 5.64 21.37 40.99 4.44 16.25 72.72 CV (%) 5.7% 7.8% 3.4% 5.3% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 274 1220 84 285 1472 Mean (pg/ml) 99 274 1220 84 285 1472 Standard deviation 5.64 21.37 40.99 4.44 16.25 72.72 Standard deviation 5.64 21.37 40.99 4.44 16.25 72.72 CV (%) 5.7% 7.8% 3.4% 5.3% 5.7% 4.9% CV (%) 5.7% 7.8% 3.4% 5.3% 5.7% 4.9%

Sensitivity

<10 pg/mL

Function

Cell surface receptor that may play a role in the innate and adaptive immune response. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

33522 MW

Specificity

Natural and recombinant mouse TREML1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Trem-like transcript 1 protein

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Sequestered in cytoplasmic vesicles in resting platelets. Transported to the cell surface after stimulation by thrombin. Soluble fragments can be released into the serum by proteolysis. .

Protein Name

Trem-like transcript 1 protein

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.