Human TREML1/Tlt 1 ELISA Kit PicoKine®

Human TREML1/Tlt 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TREML1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 32 36 35 34 1.5 4.4% Sample 2 213 199 197 200 202 6.29 3.1% Sample 3 407 453 431 394 421 22.63 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 32 36 35 34 1.5 4.4% Sample 1 35 32 36 35 34 1.5 4.4% Sample 2 213 199 197 200 202 6.29 3.1% Sample 2 213 199 197 200 202 6.29 3.1% Sample 3 407 453 431 394 421 22.63 5.3% Sample 3 407 453 431 394 421 22.63 5.3%

Synonyms

Trem-like transcript 1 protein; TLT-1; Triggering receptor expressed on myeloid cells-like protein 1; TREML1; TLT1; UNQ1825/PRO3438

Gene Name

TREML1

Gene ID

340205

UniProt

Q86YW5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q16-P162

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in platelets, monocytic leukemia and in T-cell leukemia. .

Applications

ELISA

Field of Research

Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Platelets, Protein Phosphorylation, Signal Transduction

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7%

Sensitivity

<10 pg/mL

Function

Cell surface receptor that may play a role in the innate and adaptive immune response. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32679 MW

Specificity

Natural and recombinant human TREML1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Trem-like transcript 1 protein

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Sequestered in cytoplasmic vesicles in resting platelets (PubMed:15100151) . Transported to the cell surface after stimulation by thrombin (PubMed:15100151) . Soluble fragments can be released into the serum by proteolysis (PubMed:16505478) .

Protein Name

Trem-like transcript 1 protein

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.