Mouse NOPE/IGDCC4 ELISA Kit PicoKine®

Mouse NOPE/IGDCC4 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Igdcc4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC) . Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 209 189 183 193 9.62 4.9% Sample 2 1177 1206 1298 1296 1244 53.74 4.3% Sample 3 4535 4341 3824 4624 4331 310.08 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 209 189 183 193 9.62 4.9% Sample 1 194 209 189 183 193 9.62 4.9% Sample 2 1177 1206 1298 1296 1244 53.74 4.3% Sample 2 1177 1206 1298 1296 1244 53.74 4.3% Sample 3 4535 4341 3824 4624 4331 310.08 7.1% Sample 3 4535 4341 3824 4624 4331 310.08 7.1%

Synonyms

Immunoglobulin superfamily DCC subclass member 4; Neighbor of punc e11; Protein DDM36; Igdcc4; Ddm36, Kiaa1628, Nope

Gene Name

Igdcc4

Gene ID

56741

UniProt

Q9EQS9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-H954

Tissue Specificity

Expressed in skeletal muscle, heart and brain. Brain expression is hippocampus-specific. .

Applications

ELISA

Field of Research

Cell Biology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 1177 4535 182 1179 4547 Standard deviation 13.77 63.55 184.1%3 13.83 84.88 190.97 CV (%) 7.1% 5.4% 4.1% 7.6% 7.2% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 1177 4535 182 1179 4547 Standard deviation 13.77 63.55 184.1%3 13.83 84.88 190.97 CV (%) 7.1% 5.4% 4.1% 7.6% 7.2% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 194 1177 4535 182 1179 4547 Mean (pg/ml) 194 1177 4535 182 1179 4547 Standard deviation 13.77 63.55 184.1%3 13.83 84.88 190.97 Standard deviation 13.77 63.55 184.1%3 13.83 84.88 190.97 CV (%) 7.1% 5.4% 4.1% 7.6% 7.2% 4.2% CV (%) 7.1% 5.4% 4.1% 7.6% 7.2% 4.2%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

134765 MW

Specificity

Natural and recombinant mouse NOPE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Immunoglobulin superfamily DCC subclass member 4

Subcellular Location

Cell membrane ; Single-pass type I membrane protein .

Protein Name

Immunoglobulin superfamily DCC subclass member 4

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.056 0.168 0.294 0.516 0.953 1.487 1.912 2.189 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.056 0.168 0.294 0.516 0.953 1.487 1.912 2.189 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.056 0.168 0.294 0.516 0.953 1.487 1.912 2.189 O.D. 0.056 0.168 0.294 0.516 0.953 1.487 1.912 2.189

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.