Mouse ALK-1/ACVRL1 ELISA Kit PicoKine®

Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 92 96 82 90 5.17 5.7% Sample 2 338 311 358 359 341 19.5 5.7% Sample 3 1289 1458 1378 1338 1365 61.88 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 92 96 82 90 5.17 5.7% Sample 1 92 92 96 82 90 5.17 5.7% Sample 2 338 311 358 359 341 19.5 5.7% Sample 2 338 311 358 359 341 19.5 5.7% Sample 3 1289 1458 1378 1338 1365 61.88 4.5% Sample 3 1289 1458 1378 1338 1365 61.88 4.5%

Serine/threonine-protein kinase receptor R3; SKR3;2.7.11.30; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I; Acvrl1; Acvrlk1, Alk-1

Amino Acid Metabolism, Amino Acids, Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 338 1289 84 319 1165 Standard deviation 6.8 23.66 101.83 6.63 27.43 93.2 CV (%) 7.4% 7% 7.9% 7.9% 8.6% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 338 1289 84 319 1165 Standard deviation 6.8 23.66 101.83 6.63 27.43 93.2 CV (%) 7.4% 7% 7.9% 7.9% 8.6% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 338 1289 84 319 1165 Mean (pg/ml) 92 338 1289 84 319 1165 Standard deviation 6.8 23.66 101.83 6.63 27.43 93.2 Standard deviation 6.8 23.66 101.83 6.63 27.43 93.2 CV (%) 7.4% 7% 7.9% 7.9% 8.6% 8% CV (%) 7.4% 7% 7.9% 7.9% 8.6% 8%

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.063 0.109 0.17 0.301 0.531 0.8779 1.426 2.062 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.063 0.109 0.17 0.301 0.531 0.8779 1.426 2.062 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.063 0.109 0.17 0.301 0.531 0.8779 1.426 2.062 O.D. 0.063 0.109 0.17 0.301 0.531 0.8779 1.426 2.062

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.