Human ADAM15 ELISA Kit PicoKine®

Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Sample 3 1454 1384 1331 1442 1402 49.15 3.5%

Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15;3.4.24.-; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin; ADAM15; MDC15

Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level) . Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells. .

Cancer, Cell Adhesion, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Integrins, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3%

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Endomembrane system ; Single-pass type I membrane protein . Cell junction, adherens junction . Cell projection, cilium, flagellum . Cytoplasmic vesicle, secretory vesicle, acrosome . The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed (By similarity) . .

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.