Human R-Spondin-4 ELISA Kit PicoKine®

R-spondin 4 is a protein in humans that is encoded by the RSPO4 gene. It is mapped to chromosome 20p13. This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 59 59 53 56 2.77 4.9% Sample 2 280 245 265 270 265 12.74 4.8% Sample 3 777 755 821 786 784 23.77 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 59 59 53 56 2.77 4.9% Sample 1 54 59 59 53 56 2.77 4.9% Sample 2 280 245 265 270 265 12.74 4.8% Sample 2 280 245 265 270 265 12.74 4.8% Sample 3 777 755 821 786 784 23.77 3% Sample 3 777 755 821 786 784 23.77 3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 280 777 56 262 795 Standard deviation 2.26 16.24 57.49 2.91 17.81 62.01 CV (%) 4.2% 5.8% 7.4% 5.2% 6.8% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 280 777 56 262 795 Standard deviation 2.26 16.24 57.49 2.91 17.81 62.01 CV (%) 4.2% 5.8% 7.4% 5.2% 6.8% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 280 777 56 262 795 Mean (pg/ml) 54 280 777 56 262 795 Standard deviation 2.26 16.24 57.49 2.91 17.81 62.01 Standard deviation 2.26 16.24 57.49 2.91 17.81 62.01 CV (%) 4.2% 5.8% 7.4% 5.2% 6.8% 7.8% CV (%) 4.2% 5.8% 7.4% 5.2% 6.8% 7.8%

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.216 0.328 0.496 0.763 1.229 1.912 2.879 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.216 0.328 0.496 0.763 1.229 1.912 2.879 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.216 0.328 0.496 0.763 1.229 1.912 2.879 O.D. 0.056 0.216 0.328 0.496 0.763 1.229 1.912 2.879

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.