Human Fetuin B/FETUB ELISA Kit PicoKine®

Fetuin-B is a protein that in humans is encoded by the FETUB gene. It is mapped to 3q27, where the FETUA gene is localized. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Sample 3 4549 4286 4769 4692 4574 184.06 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8%

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.