Mouse Persephin ELISA Kit PicoKine®

Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3.849 3.865 3.812 53.79 1.4% Sample 2 12.058 12.135 11.622 10.357 11.543 712.13 6.1% Sample 3 49.927 48.196 52.754 50.855 50.433 1645.09 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3.849 3.865 3.812 53.79 1.4% Sample 1 3726 3810 3.849 3.865 3.812 53.79 1.4% Sample 2 12.058 12.135 11.622 10.357 11.543 712.13 6.1% Sample 2 12.058 12.135 11.622 10.357 11.543 712.13 6.1% Sample 3 49.927 48.196 52.754 50.855 50.433 1645.09 3.2% Sample 3 49.927 48.196 52.754 50.855 50.433 1645.09 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3.7 12 49 3.6 11.4 55 Standard deviation 0.18 0.94 2.3 0.23 1.026 2.86 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3.7 12 49 3.6 11.4 55 Standard deviation 0.18 0.94 2.3 0.23 1.026 2.86 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3.7 12 49 3.6 11.4 55 Mean (pg/ml) 3.7 12 49 3.6 11.4 55 Standard deviation 0.18 0.94 2.3 0.23 1.026 2.86 Standard deviation 0.18 0.94 2.3 0.23 1.026 2.86 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.298 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.298 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.298 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.298

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.