Human Persephin ELISA Kit PicoKine®

Human Persephin ELISA Kit PicoKine® (96 Tests) . Quantitate Human PSPN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 175 184 206 197 190 11.88 6.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 3 3887 4123 4146 3946 4025 111.27 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 175 184 206 197 190 11.88 6.2% Sample 1 175 184 206 197 190 11.88 6.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 3 3887 4123 4146 3946 4025 111.27 2.7% Sample 3 3887 4123 4146 3946 4025 111.27 2.7%

Synonyms

Persephin; PSP; PSPN

Gene Name

PSPN

Gene ID

5623

UniProt

O60542

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: W22-G156

Applications

ELISA

Field of Research

Growth and Development, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8%

Sensitivity

<10 pg/mL

Function

Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

16600 MW

Specificity

Natural and recombinant human Persephin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Persephin

Subcellular Location

Secreted.

Protein Name

Persephin

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.