Human EDIL3 ELISA Kit PicoKine®

EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV) /beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 36 57 60 52 3.62 6.9% Sample 2 181 196 202 205 196 6.25 3.2% Sample 3 804 970 736 552 766 35.37 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 36 57 60 52 3.62 6.9% Sample 1 56 36 57 60 52 3.62 6.9% Sample 2 181 196 202 205 196 6.25 3.2% Sample 2 181 196 202 205 196 6.25 3.2% Sample 3 804 970 736 552 766 35.37 4.6% Sample 3 804 970 736 552 766 35.37 4.6%

EGF-like repeat and discoidin I-like domain-containing protein 3; Developmentally-regulated endothelial cell locus 1 protein; Integrin-binding protein DEL1; EDIL3; DEL1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 181 804 59 203 803 Standard deviation 1.52 7.17 30.55 1.4 5.22 60.47 CV (%) 2.7% 4% 3.8% 2.4% 2.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 181 804 59 203 803 Standard deviation 1.52 7.17 30.55 1.4 5.22 60.47 CV (%) 2.7% 4% 3.8% 2.4% 2.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 181 804 59 203 803 Mean (pg/ml) 56 181 804 59 203 803 Standard deviation 1.52 7.17 30.55 1.4 5.22 60.47 Standard deviation 1.52 7.17 30.55 1.4 5.22 60.47 CV (%) 2.7% 4% 3.8% 2.4% 2.6% 7.5% CV (%) 2.7% 4% 3.8% 2.4% 2.6% 7.5%

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.136 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.136 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.136 0.201 0.348 0.6254 1.135 1.946 O.D. 0.056 0.099 0.136 0.201 0.348 0.6254 1.135 1.946

Aliquot 0.1 ml per well of the 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml human EDIL3 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well) . Add 0.1 ml of each properly diluted sample of human cell culture supernatants, cell lysates, serum or plasma (heparin, EDTA) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each human EDIL3 standard solution and each sample be measured in duplicate.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.