Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 530 551 501 466 512 31.94 6.2% Sample 2 1744 1935 1605 1844 1782 122.5 6.8% Sample 3 8630 9352 9453 8421 8964 446.11 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 530 551 501 466 512 31.94 6.2% Sample 1 530 551 501 466 512 31.94 6.2% Sample 2 1744 1935 1605 1844 1782 122.5 6.8% Sample 2 1744 1935 1605 1844 1782 122.5 6.8% Sample 3 8630 9352 9453 8421 8964 446.11 4.9% Sample 3 8630 9352 9453 8421 8964 446.11 4.9%

Cancer, Cancer Metabolism, G Protein Signaling, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 530 1744 8630 561 1890 8353 Standard deviation 25.44 104.64 576.1%%1 35.34 114.8%9 651.53 CV (%) 4.8% 6% 6.7% 6.1%% 6.1% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 530 1744 8630 561 1890 8353 Standard deviation 25.44 104.64 576.1%%1 35.34 114.8%9 651.53 CV (%) 4.8% 6% 6.7% 6.1%% 6.1% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 530 1744 8630 561 1890 8353 Mean (pg/ml) 530 1744 8630 561 1890 8353 Standard deviation 25.44 104.64 576.1%%1 35.34 114.8%9 651.53 Standard deviation 25.44 104.64 576.1%%1 35.34 114.8%9 651.53 CV (%) 4.8% 6% 6.7% 6.1%% 6.1% 7.8% CV (%) 4.8% 6% 6.7% 6.1%% 6.1% 7.8%

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.044 0.079 0.169 0.347 0.68 1.442 2.317 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.044 0.079 0.169 0.347 0.68 1.442 2.317 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.044 0.079 0.169 0.347 0.68 1.442 2.317 O.D. 0.005 0.044 0.079 0.169 0.347 0.68 1.442 2.317

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.