Human LRIG3 ELISA Kit PicoKine®

LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 108 99 93 100 5.33 5.3% Sample 2 680 623 576 637 629 37.11 5.8% Sample 3 1076 1073 1250 1113 1128 72.17 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 108 99 93 100 5.33 5.3% Sample 1 100 108 99 93 100 5.33 5.3% Sample 2 680 623 576 637 629 37.11 5.8% Sample 2 680 623 576 637 629 37.11 5.8% Sample 3 1076 1073 1250 1113 1128 72.17 6.3% Sample 3 1076 1073 1250 1113 1128 72.17 6.3%

Auditory System, Cancer, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Intracellular, Neuroscience, Sensory System, Stem Cells, Surface Molecules, Tumor Antigens, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 680 1076 92 689 1148 Standard deviation 4.9 46.92 73.16 5.79 48.91 89.54 CV (%) 4.9% 6.9% 6.8% 7.1%% 7.1% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 680 1076 92 689 1148 Standard deviation 4.9 46.92 73.16 5.79 48.91 89.54 CV (%) 4.9% 6.9% 6.8% 7.1%% 7.1% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 680 1076 92 689 1148 Mean (pg/ml) 100 680 1076 92 689 1148 Standard deviation 4.9 46.92 73.16 5.79 48.91 89.54 Standard deviation 4.9 46.92 73.16 5.79 48.91 89.54 CV (%) 4.9% 6.9% 6.8% 7.1%% 7.1% 7.8% CV (%) 4.9% 6.9% 6.8% 7.1%% 7.1% 7.8%

May play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Detected in cytoplasmic vesicles when coexpressed with ERBB4. .

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.069 0.161 0.359 0.67 1.288 1.955 2.224 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.069 0.161 0.359 0.67 1.288 1.955 2.224 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.069 0.161 0.359 0.67 1.288 1.955 2.224 O.D. 0.007 0.069 0.161 0.359 0.67 1.288 1.955 2.224

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.