Rat GDF5/Bmp 14 ELISA Kit PicoKine®

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 59 68 62 62 3.49 5.6% Sample 2 323 317 316 281 309 16.52 5.3% Sample 3 894 859 910 831 873 30.69 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 59 68 62 62 3.49 5.6% Sample 1 60 59 68 62 62 3.49 5.6% Sample 2 323 317 316 281 309 16.52 5.3% Sample 2 323 317 316 281 309 16.52 5.3% Sample 3 894 859 910 831 873 30.69 3.5% Sample 3 894 859 910 831 873 30.69 3.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.