Mouse GDF5/Bmp 14 ELISA Kit PicoKine®

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 70 77 78 73 4.03 5.5% Sample 2 222 259 247 228 239 14.78 6.1% Sample 3 1053 912 969 1030 991 54.97 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 70 77 78 73 4.03 5.5% Sample 1 69 70 77 78 73 4.03 5.5% Sample 2 222 259 247 228 239 14.78 6.1% Sample 2 222 259 247 228 239 14.78 6.1% Sample 3 1053 912 969 1030 991 54.97 5.5% Sample 3 1053 912 969 1030 991 54.97 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 222 1053 62 217 1005 Standard deviation 4.34 16%8 63.18 4.89 17.14 62.31 CV (%) 7.9%% 7.2% 6% 7.9% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 222 1053 62 217 1005 Standard deviation 4.34 16%8 63.18 4.89 17.14 62.31 CV (%) 7.9%% 7.2% 6% 7.9% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 222 1053 62 217 1005 Mean (pg/ml) 69 222 1053 62 217 1005 Standard deviation 4.34 16%8 63.18 4.89 17.14 62.31 Standard deviation 4.34 16%8 63.18 4.89 17.14 62.31 CV (%) 7.9%% 7.2% 6% 7.9% 7.9% 6.2% CV (%) 7.9%% 7.2% 6% 7.9% 7.9% 6.2%

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (By similarity) . Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (By similarity) . Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (PubMed:24076600) . Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.089 0.155 0.26 0.474 0.776 1.343 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.089 0.155 0.26 0.474 0.776 1.343 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.089 0.155 0.26 0.474 0.776 1.343 2.05 O.D. 0.019 0.089 0.155 0.26 0.474 0.776 1.343 2.05

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.