Human TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine®

Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Sample 3 4549 4286 4769 4692 4574 184.06 4%

Amino Acid Metabolism, Amino Acids, Atherosclerosis, Cardiovascular, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8%

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.