Human Siglec-7/CD328 ELISA Kit PicoKine®

Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 199 198 204 193 11.62 6% Sample 2 1303 1333 1368 1214 1304 57.08 4.3% Sample 3 3795 3634 4070 3787 3821 157.16 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 199 198 204 193 11.62 6% Sample 1 174 199 198 204 193 11.62 6% Sample 2 1303 1333 1368 1214 1304 57.08 4.3% Sample 2 1303 1333 1368 1214 1304 57.08 4.3% Sample 3 3795 3634 4070 3787 3821 157.16 4.1% Sample 3 3795 3634 4070 3787 3821 157.16 4.1%

Sialic acid-binding Ig-like lectin 7; Siglec-7; Adhesion inhibitory receptor molecule 1; AIRM-1; CDw328; D-siglec; QA79 membrane protein; p75; CD328; SIGLEC7; AIRM1

Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression found in placenta, liver, lung, spleen, and peripheral blood leukocytes.

Cardiovascular, Cell Type Marker, Hematopoietic Progenitors, Immunology, Innate Immunity, Neuron Marker, Neuroscience, NK Cells, Stem Cells, Surface Molecules

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 1303 3179 179 1384 3570 Standard deviation 7.83 93.81 238.43 8.41 123.17 224.91 CV (%) 4.5% 7.2% 7.5% 4.7% 8.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 1303 3179 179 1384 3570 Standard deviation 7.83 93.81 238.43 8.41 123.17 224.91 CV (%) 4.5% 7.2% 7.5% 4.7% 8.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 174 1303 3179 179 1384 3570 Mean (pg/ml) 174 1303 3179 179 1384 3570 Standard deviation 7.83 93.81 238.43 8.41 123.17 224.91 Standard deviation 7.83 93.81 238.43 8.41 123.17 224.91 CV (%) 4.5% 7.2% 7.5% 4.7% 8.9% 6.3% CV (%) 4.5% 7.2% 7.5% 4.7% 8.9% 6.3%

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide) . The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.007 0.041 0.066 0.135 0.274 0.5625 1.285 2.437 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.007 0.041 0.066 0.135 0.274 0.5625 1.285 2.437 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.007 0.041 0.066 0.135 0.274 0.5625 1.285 2.437 O.D. 0.007 0.041 0.066 0.135 0.274 0.5625 1.285 2.437

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.