Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 206 209 222 189 206 11.75 5.7% Sample 2 1289 1163 1209 1152 1203 53.92 4.4% Sample 3 4332 4412 4054 4654 4363 214.19 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 206 209 222 189 206 11.75 5.7% Sample 1 206 209 222 189 206 11.75 5.7% Sample 2 1289 1163 1209 1152 1203 53.92 4.4% Sample 2 1289 1163 1209 1152 1203 53.92 4.4% Sample 3 4332 4412 4054 4654 4363 214.19 4.9% Sample 3 4332 4412 4054 4654 4363 214.19 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 206 1289 4332 201 1293 3972 Standard deviation 8.44 68.31 316.23 11.65 68.52 317.76 CV (%) 4.1% 5.3% 7.3% 5.8% 5.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 206 1289 4332 201 1293 3972 Standard deviation 8.44 68.31 316.23 11.65 68.52 317.76 CV (%) 4.1% 5.3% 7.3% 5.8% 5.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 206 1289 4332 201 1293 3972 Mean (pg/ml) 206 1289 4332 201 1293 3972 Standard deviation 8.44 68.31 316.23 11.65 68.52 317.76 Standard deviation 8.44 68.31 316.23 11.65 68.52 317.76 CV (%) 4.1% 5.3% 7.3% 5.8% 5.3% 8% CV (%) 4.1% 5.3% 7.3% 5.8% 5.3% 8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.106 0.198 0.356 0.664 1.134 1.43 2.035 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.106 0.198 0.356 0.664 1.134 1.43 2.035 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.106 0.198 0.356 0.664 1.134 1.43 2.035 O.D. 0.054 0.106 0.198 0.356 0.664 1.134 1.43 2.035

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.