Human R-Spondin-3 ELISA Kit PicoKine®

R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family) /beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 34 29 32 32 2.04 6.3% Sample 2 151 180 171 161 165 10.84 6.5% Sample 3 397 452 458 408 428 26.62 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 34 29 32 32 2.04 6.3% Sample 1 34 34 29 32 32 2.04 6.3% Sample 2 151 180 171 161 165 10.84 6.5% Sample 2 151 180 171 161 165 10.84 6.5% Sample 3 397 452 458 408 428 26.62 6.2% Sample 3 397 452 458 408 428 26.62 6.2%

R-spondin-3; Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2; RSPO3; PWTSR, THSD2

Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node (PubMed:12463421) . Highly expressed in endothelial cells (PubMed:26766444) . .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 151 397 31 138 371 Standard deviation 1.49 6.04 27.79 1.55 8.14 25.9%4 CV (%) 4.4% 4% 7% 5% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 151 397 31 138 371 Standard deviation 1.49 6.04 27.79 1.55 8.14 25.9%4 CV (%) 4.4% 4% 7% 5% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 151 397 31 138 371 Mean (pg/ml) 34 151 397 31 138 371 Standard deviation 1.49 6.04 27.79 1.55 8.14 25.9%4 Standard deviation 1.49 6.04 27.79 1.55 8.14 25.9%4 CV (%) 4.4% 4% 7% 5% 5.9% 7.1% CV (%) 4.4% 4% 7% 5% 5.9% 7.1%

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta- catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:21727895, PubMed:21909076, PubMed:22615920) . Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.111 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.111 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.111 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.111

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.