Human Galectin-1 ELISA Kit PicoKine®

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 226 230 221 227 4.2 1.8% Sample 2 1786 1592 1746 1838 1740 91.73 5.2% Sample 3 5647 5665 6073 5299 5671 274.13 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 226 230 221 227 4.2 1.8% Sample 1 232 226 230 221 227 4.2 1.8% Sample 2 1786 1592 1746 1838 1740 91.73 5.2% Sample 2 1786 1592 1746 1838 1740 91.73 5.2% Sample 3 5647 5665 6073 5299 5671 274.13 4.8% Sample 3 5647 5665 6073 5299 5671 274.13 4.8%

Galectin-1; Gal-1;14 kDa laminin-binding protein; HLBP14;14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1; LGALS1

Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level) . Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 1786 5647 229 1822 5807 Standard deviation 13.45 121.44 355.76 13.96 147.58 476.17 CV (%) 5.8% 6.8% 8.1% 6.1% 8.1% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 1786 5647 229 1822 5807 Standard deviation 13.45 121.44 355.76 13.96 147.58 476.17 CV (%) 5.8% 6.8% 8.1% 6.1% 8.1% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 232 1786 5647 229 1822 5807 Mean (pg/ml) 232 1786 5647 229 1822 5807 Standard deviation 13.45 121.44 355.76 13.96 147.58 476.17 Standard deviation 13.45 121.44 355.76 13.96 147.58 476.17 CV (%) 5.8% 6.8% 8.1% 6.1% 8.1% 8.2% CV (%) 5.8% 6.8% 8.1% 6.1% 8.1% 8.2%

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.136 0.203 0.306 0.564 0.965 1.571 2.08 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.136 0.203 0.306 0.564 0.965 1.571 2.08 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.136 0.203 0.306 0.564 0.965 1.571 2.08 O.D. 0.049 0.136 0.203 0.306 0.564 0.965 1.571 2.08

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.