Mouse TPOR/MPL/Thrombopoietin R ELISA Kit PicoKine®

The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 290 290 302 298 8.48 2.8% Sample 2 1660 1604 1470 1495 1557 77.83 4.9% Sample 3 3993 4149 4386 4522 4262 205 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 290 290 302 298 8.48 2.8% Sample 1 310 290 290 302 298 8.48 2.8% Sample 2 1660 1604 1470 1495 1557 77.83 4.9% Sample 2 1660 1604 1470 1495 1557 77.83 4.9% Sample 3 3993 4149 4386 4522 4262 205 4.8% Sample 3 3993 4149 4386 4522 4262 205 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1660 3993 319 1771 4235 Standard deviation 15.6%9 131.14 171.69 18.82 143.45 237.16 CV (%) 4.9% 7.9% 4.3% 5.9% 8.1% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1660 3993 319 1771 4235 Standard deviation 15.6%9 131.14 171.69 18.82 143.45 237.16 CV (%) 4.9% 7.9% 4.3% 5.9% 8.1% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1660 3993 319 1771 4235 Mean (pg/ml) 310 1660 3993 319 1771 4235 Standard deviation 15.6%9 131.14 171.69 18.82 143.45 237.16 Standard deviation 15.6%9 131.14 171.69 18.82 143.45 237.16 CV (%) 4.9% 7.9% 4.3% 5.9% 8.1% 5.6% CV (%) 4.9% 7.9% 4.3% 5.9% 8.1% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.