Human Fibulin-3/EFEMP1 ELISA Kit PicoKine®

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 650 662 670 638 655 12.12 1.8% Sample 2 2080 2103 2020 2397 2150 145.78 6.7% Sample 3 10283 8683 9084 9862 9478 629.03 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 650 662 670 638 655 12.12 1.8% Sample 1 650 662 670 638 655 12.12 1.8% Sample 2 2080 2103 2020 2397 2150 145.78 6.7% Sample 2 2080 2103 2020 2397 2150 145.78 6.7% Sample 3 10283 8683 9084 9862 9478 629.03 6.6% Sample 3 10283 8683 9084 9862 9478 629.03 6.6%

In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 650 2080 10283 629 2055 10529 Standard deviation 31.2 84.88 473.01 37.11 117.13 610.68 CV (%) 4.8% 4.1% 4.6% 5.9% 5.7% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 650 2080 10283 629 2055 10529 Standard deviation 31.2 84.88 473.01 37.11 117.13 610.68 CV (%) 4.8% 4.1% 4.6% 5.9% 5.7% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 650 2080 10283 629 2055 10529 Mean (pg/ml) 650 2080 10283 629 2055 10529 Standard deviation 31.2 84.88 473.01 37.11 117.13 610.68 Standard deviation 31.2 84.88 473.01 37.11 117.13 610.68 CV (%) 4.8% 4.1% 4.6% 5.9% 5.7% 5.8% CV (%) 4.8% 4.1% 4.6% 5.9% 5.7% 5.8%

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted, extracellular space . Secreted, extracellular space, extracellular matrix . Localizes to the lamina propria underneath the olfactory epithelium. .

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.037 0.061 0.085 0.147 0.289 0.537 1.066 2.2 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.037 0.061 0.085 0.147 0.289 0.537 1.066 2.2 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.037 0.061 0.085 0.147 0.289 0.537 1.066 2.2 O.D. 0.037 0.061 0.085 0.147 0.289 0.537 1.066 2.2

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.