Human GHR/Growth Hormone R ELISA Kit PicoKine®

Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to chromosome 5p13.1-p12. This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization (the receptor may however also exist as a pre-assembled non-functional dimer) and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 353 349 346 304 338 19.78 5.8% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 3 4591 4345 4168 3982 4271 224.72 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 353 349 346 304 338 19.78 5.8% Sample 1 353 349 346 304 338 19.78 5.8% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 3 4591 4345 4168 3982 4271 224.72 5.2% Sample 3 4591 4345 4168 3982 4271 224.72 5.2%

Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2%

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Single-pass type I membrane protein. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. .

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.