Human Hemopexin ELISA Kit PicoKine®

Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7144 6667 7002 7690 7125 368.94 5.1% Sample 2 26562 26917 29717 26482 27419 1336.52 4.8% Sample 3 114865 125689 107714 123178 117861 7097.28 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7144 6667 7002 7690 7125 368.94 5.1% Sample 1 7144 6667 7002 7690 7125 368.94 5.1% Sample 2 26562 26917 29717 26482 27419 1336.52 4.8% Sample 2 26562 26917 29717 26482 27419 1336.52 4.8% Sample 3 114865 125689 107714 123178 117861 7097.28 6% Sample 3 114865 125689 107714 123178 117861 7097.28 6%

Cardiovascular, Cofactors, Vitamins/Minerals, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7144 26562 114865 7626 24479 107968 Standard deviation 535.8 1089.04 5628.38 571.95 1345.5%4 7125.88 CV (%) 7.5% 4.1% 4.9% 7.5% 5.5% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7144 26562 114865 7626 24479 107968 Standard deviation 535.8 1089.04 5628.38 571.95 1345.5%4 7125.88 CV (%) 7.5% 4.1% 4.9% 7.5% 5.5% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 7144 26562 114865 7626 24479 107968 Mean (pg/ml) 7144 26562 114865 7626 24479 107968 Standard deviation 535.8 1089.04 5628.38 571.95 1345.5%4 7125.88 Standard deviation 535.8 1089.04 5628.38 571.95 1345.5%4 7125.88 CV (%) 7.5% 4.1% 4.9% 7.5% 5.5% 6.6% CV (%) 7.5% 4.1% 4.9% 7.5% 5.5% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.08 0.119 0.219 0.365 0.733 1.331 2.196 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.08 0.119 0.219 0.365 0.733 1.331 2.196 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.08 0.119 0.219 0.365 0.733 1.331 2.196 O.D. 0.028 0.08 0.119 0.219 0.365 0.733 1.331 2.196

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.