Human HE4 ELISA Kit PicoKine®

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 246 276 254 281 264 14.63 5.5% Sample 2 1139 1088 1273 1192 1173 68.45 5.8% Sample 3 4402 3964 4189 4282 4209 160.47 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 246 276 254 281 264 14.63 5.5% Sample 1 246 276 254 281 264 14.63 5.5% Sample 2 1139 1088 1273 1192 1173 68.45 5.8% Sample 2 1139 1088 1273 1192 1173 68.45 5.8% Sample 3 4402 3964 4189 4282 4209 160.47 3.8% Sample 3 4402 3964 4189 4282 4209 160.47 3.8%

WAP four-disulfide core domain protein 2; Epididymal secretory protein E4; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WFDC2; HE4, WAP5

Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 246 1139 4402 255 1184 4098 Standard deviation 14.02 77.45 176.08 18.62 101.82 176.21 CV (%) 5.7% 6.8% 4% 6.4% 8.6% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 246 1139 4402 255 1184 4098 Standard deviation 14.02 77.45 176.08 18.62 101.82 176.21 CV (%) 5.7% 6.8% 4% 6.4% 8.6% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 246 1139 4402 255 1184 4098 Mean (pg/ml) 246 1139 4402 255 1184 4098 Standard deviation 14.02 77.45 176.08 18.62 101.82 176.21 Standard deviation 14.02 77.45 176.08 18.62 101.82 176.21 CV (%) 5.7% 6.8% 4% 6.4% 8.6% 4.3% CV (%) 5.7% 6.8% 4% 6.4% 8.6% 4.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.