Mouse FCRN/FCGRT ELISA Kit PicoKine®

The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine, TNF-α, and down-regulated by IFN-γ.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 123 130 122 126 4.32 3.4% Sample 2 1019 1070 915 953 989 59.65 6% Sample 3 2423 2361 2207 2520 2377 113.7 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 123 130 122 126 4.32 3.4% Sample 1 132 123 130 122 126 4.32 3.4% Sample 2 1019 1070 915 953 989 59.65 6% Sample 2 1019 1070 915 953 989 59.65 6% Sample 3 2423 2361 2207 2520 2377 113.7 4.7% Sample 3 2423 2361 2207 2520 2377 113.7 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 1019 2423 143 1117 2272 Standard deviation 4%8 71.33 118.72 7.57 99.41 134.04 CV (%) 4% 7% 4.9% 5.3% 8.9% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 1019 2423 143 1117 2272 Standard deviation 4%8 71.33 118.72 7.57 99.41 134.04 CV (%) 4% 7% 4.9% 5.3% 8.9% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 1019 2423 143 1117 2272 Mean (pg/ml) 132 1019 2423 143 1117 2272 Standard deviation 4%8 71.33 118.72 7.57 99.41 134.04 Standard deviation 4%8 71.33 118.72 7.57 99.41 134.04 CV (%) 4% 7% 4.9% 5.3% 8.9% 5.9% CV (%) 4% 7% 4.9% 5.3% 8.9% 5.9%

Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.073 0.128 0.247 0.461 0.87 1.686 2.529 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.073 0.128 0.247 0.461 0.87 1.686 2.529 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.073 0.128 0.247 0.461 0.87 1.686 2.529 O.D. 0.014 0.073 0.128 0.247 0.461 0.87 1.686 2.529

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.