Human Marapsin/Pancresin ELISA Kit PicoKine®

Human Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests) . Quantitate Human PRSS27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 176 171 165 169 4.2 2.4% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 3 5662 5287 5308 6068 5581 318.07 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 176 171 165 169 4.2 2.4% Sample 1 167 176 171 165 169 4.2 2.4% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 3 5662 5287 5308 6068 5581 318.07 5.6% Sample 3 5662 5287 5308 6068 5581 318.07 5.6%

Synonyms

Serine protease 27;3.4.21.-; Marapsin; Pancreasin; PRSS27; MPN; UNQ1884/PRO4327

Gene Name

PRSS27

Gene ID

83886

UniProt

Q9BQR3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M35-K290

Tissue Specificity

Expressed predominantly in the pancreas. .

Applications

ELISA

Field of Research

Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31940 MW

Specificity

Natural and recombinant human Marapsin

Gene Name Synonym

Serine protease 27

Subcellular Location

Secreted.

Protein Name

Serine protease 27

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.