Rat MMP-9 ELISA Kit PicoKine®

Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB) . The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 429 457 420 449 438 14.87 3.3% Sample 2 2362 2453 2332 2307 2363 55.22 2.3% Sample 3 8880 10067 9013 9434 9348 462.49 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 429 457 420 449 438 14.87 3.3% Sample 1 429 457 420 449 438 14.87 3.3% Sample 2 2362 2453 2332 2307 2363 55.22 2.3% Sample 2 2362 2453 2332 2307 2363 55.22 2.3% Sample 3 8880 10067 9013 9434 9348 462.49 4.9% Sample 3 8880 10067 9013 9434 9348 462.49 4.9%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 429 2362 8880 450 2199 8392 Standard deviation 29.17 153.53 594.96 33.75 167.12 646.18 CV (%) 6.8% 6.5% 6.7% 7.5% 7.6% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 429 2362 8880 450 2199 8392 Standard deviation 29.17 153.53 594.96 33.75 167.12 646.18 CV (%) 6.8% 6.5% 6.7% 7.5% 7.6% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 429 2362 8880 450 2199 8392 Mean (pg/ml) 429 2362 8880 450 2199 8392 Standard deviation 29.17 153.53 594.96 33.75 167.12 646.18 Standard deviation 29.17 153.53 594.96 33.75 167.12 646.18 CV (%) 6.8% 6.5% 6.7% 7.5% 7.6% 7.7% CV (%) 6.8% 6.5% 6.7% 7.5% 7.6% 7.7%

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.092 0.175 0.217 0.302 0.504 0.812 1.321 1.984 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.092 0.175 0.217 0.302 0.504 0.812 1.321 1.984 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.092 0.175 0.217 0.302 0.504 0.812 1.321 1.984 O.D. 0.092 0.175 0.217 0.302 0.504 0.812 1.321 1.984

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.