Human CA125/MUC16 ELISA Kit PicoKine®

Human CA125/MUC16 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MUC16 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 1.5 U/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.

Reproducibility

Lots Lot 1 (U/ml) Lot 2 (U/ml) Lot 3 (U/ml) Lot 4 (U/ml) Mean (U/ml) Standard Deviation CV (%) Sample 1 6 7 7 7 7 0.41 6.8% Sample 2 8 8 11 11 10 0.31 3.3% Sample 3 71 64 98 79 78 2.56 3.3% Lots Lot 1 (U/ml) Lot 2 (U/ml) Lot 3 (U/ml) Lot 4 (U/ml) Mean (U/ml) Standard Deviation CV (%) Lots Lot 1 (U/ml) Lot 2 (U/ml) Lot 3 (U/ml) Lot 4 (U/ml) Mean (U/ml) Standard Deviation CV (%) Sample 1 6 7 7 7 7 0.41 6.8% Sample 1 6 7 7 7 7 0.41 6.8% Sample 2 8 8 11 11 10 0.31 3.3% Sample 2 8 8 11 11 10 0.31 3.3% Sample 3 71 64 98 79 78 2.56 3.3% Sample 3 71 64 98 79 78 2.56 3.3%

Synonyms

Mucin-16 ; MUC-16; Ovarian cancer-related tumor marker CA125 ; CA-125 ; Ovarian carcinoma antigen CA125 ; MUC16 ; CA125

Gene Name

MUC16

Gene ID

94025

UniProt

Q8WXI7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Natural protein

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in corneal and conjunctival epithelia (at protein level) . Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. .

Applications

ELISA

Field of Research

Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Standard

200 U/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

3.12 U/mL - 200 U/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (U/ml) 6 8 71 4 10 77 Standard deviation 0.42 0.63 1.94 0.28 0.7 6.04 CV (%) 7% 7.9% 2.7% 7% 7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (U/ml) 6 8 71 4 10 77 Standard deviation 0.42 0.63 1.94 0.28 0.7 6.04 CV (%) 7% 7.9% 2.7% 7% 7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (U/ml) 6 8 71 4 10 77 Mean (U/ml) 6 8 71 4 10 77 Standard deviation 0.42 0.63 1.94 0.28 0.7 6.04 Standard deviation 0.42 0.63 1.94 0.28 0.7 6.04 CV (%) 7% 7.9% 2.7% 7% 7% 7.8% CV (%) 7% 7.9% 2.7% 7% 7% 7.8%

Sensitivity

<1.5 U/mL

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

1519175 MW

Specificity

Natural and recombinant human CA125

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Mucin-16

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Protein Name

Mucin-16

Sample Data

Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.056 0.099 0.142 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.056 0.099 0.142 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.056 0.099 0.142 0.201 0.348 0.6254 1.135 1.946 O.D. 0.056 0.099 0.142 0.201 0.348 0.6254 1.135 1.946

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.