Human SAA/SAA1 ELISA Kit PicoKine®

Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3858 3499 3839 4125 3830 222.17 5.8% Sample 2 13218 13736 13026 14070 13512 413.57 3% Sample 3 48138 46567 52708 53611 50256 2973.33 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3858 3499 3839 4125 3830 222.17 5.8% Sample 1 3858 3499 3839 4125 3830 222.17 5.8% Sample 2 13218 13736 13026 14070 13512 413.57 3% Sample 2 13218 13736 13026 14070 13512 413.57 3% Sample 3 48138 46567 52708 53611 50256 2973.33 5.9% Sample 3 48138 46567 52708 53611 50256 2973.33 5.9%

Serum amyloid A-1 protein; SAA; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid protein A (2-104) ; Serum amyloid protein A (3-104) ; Serum amyloid protein A (2-103) ; Serum amyloid protein A (2-102) ; Serum amyloid protein A (4-101) ; SAA1

Cardiovascular, Complement, Cytokines, G Protein Signaling, Immunology, Innate Immunity, Lipids/Lipoproteins, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3858 13218 48138 4235 13787 47081 Standard deviation 293.2 581.59 2406.9 393.85 868.58 2777.77 CV (%) 7.6% 4.4% 5% 9.3% 6.3% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3858 13218 48138 4235 13787 47081 Standard deviation 293.2 581.59 2406.9 393.85 868.58 2777.77 CV (%) 7.6% 4.4% 5% 9.3% 6.3% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3858 13218 48138 4235 13787 47081 Mean (pg/ml) 3858 13218 48138 4235 13787 47081 Standard deviation 293.2 581.59 2406.9 393.85 868.58 2777.77 Standard deviation 293.2 581.59 2406.9 393.85 868.58 2777.77 CV (%) 7.6% 4.4% 5% 9.3% 6.3% 5.9% CV (%) 7.6% 4.4% 5% 9.3% 6.3% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.032 0.081 0.163 0.292 0.497 0.904 1.478 1.992 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.032 0.081 0.163 0.292 0.497 0.904 1.478 1.992 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.032 0.081 0.163 0.292 0.497 0.904 1.478 1.992 O.D. 0.032 0.081 0.163 0.292 0.497 0.904 1.478 1.992

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.