Human Oncomodulin ELISA Kit PicoKine®

Human Oncomodulin ELISA Kit PicoKine® (96 Tests) . Quantitate Human OCM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Oncomodulin, also known as OCM, is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. The human gene for oncomodulin is located on chromosome 7. It is a parvalbumin- family calcium-binding protein expressed and secreted by macrophages that typically traffic to tissue as an inflammatory response or after injury. Oncomodulin promotes axon regeneration in retinal ganglion cells and maintains functioning in mouse cochlear hair cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 101 94 100 99 3.11 3.1% Sample 2 272 264 287 282 276 8.89 3.2% Sample 3 897 877 847 824 861 27.91 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 101 94 100 99 3.11 3.1% Sample 1 102 101 94 100 99 3.11 3.1% Sample 2 272 264 287 282 276 8.89 3.2% Sample 2 272 264 287 282 276 8.89 3.2% Sample 3 897 877 847 824 861 27.91 3.2% Sample 3 897 877 847 824 861 27.91 3.2%

Synonyms

Oncomodulin-1; OM; Parvalbumin beta; OCM; OCM1, OCMN

Gene Name

OCM

Gene ID

654231

UniProt

P0CE72

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-S109

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Calcium Binding Proteins, Calcium Signaling, Signal Transduction, Signaling Pathway

Standard

2 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 272 897 105 292 975 Standard deviation 5.5 20.67 63.68 7.14 25.4 85.8 CV (%) 5.4% 7.6% 7.1% 6.8% 8.7% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 272 897 105 292 975 Standard deviation 5.5 20.67 63.68 7.14 25.4 85.8 CV (%) 5.4% 7.6% 7.1% 6.8% 8.7% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 272 897 105 292 975 Mean (pg/ml) 102 272 897 105 292 975 Standard deviation 5.5 20.67 63.68 7.14 25.4 85.8 Standard deviation 5.5 20.67 63.68 7.14 25.4 85.8 CV (%) 5.4% 7.6% 7.1% 6.8% 8.7% 8.8% CV (%) 5.4% 7.6% 7.1% 6.8% 8.7% 8.8%

Sensitivity

<10 pg/mL

Function

Has some calmodulin-like activity with respect to enzyme activation and growth regulation. Binds two calcium ions.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12184 MW

Specificity

Natural and recombinant human Oncomodulin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Oncomodulin-1

Protein Name

Oncomodulin-1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.084 0.144 0.25 0.466 0.895 1.607 2.736 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.084 0.144 0.25 0.466 0.895 1.607 2.736 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.084 0.144 0.25 0.466 0.895 1.607 2.736 O.D. 0.015 0.084 0.144 0.25 0.466 0.895 1.607 2.736

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.