Mouse BCAM/Cd239 ELISA Kit PicoKine®

Mouse BCAM/Cd239 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Bcam in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 99 112 96 103 3.4 3.3% Sample 2 288 265 272 281 277 8.57 3.1% Sample 3 896 845 826 881 862 30.17 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 99 112 96 103 3.4 3.3% Sample 1 105 99 112 96 103 3.4 3.3% Sample 2 288 265 272 281 277 8.57 3.1% Sample 2 288 265 272 281 277 8.57 3.1% Sample 3 896 845 826 881 862 30.17 3.5% Sample 3 896 845 826 881 862 30.17 3.5%

Synonyms

Basal cell adhesion molecule; B-CAM cell surface glycoprotein; Lutheran antigen; CD239; Bcam; Gplu, Lu

Gene Name

Bcam

Gene ID

57278

UniProt

Q9R069

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E26-A541

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3%

Sensitivity

<15 pg/mL

Function

Laminin alpha-5 receptor. May mediate intracellular signaling (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

67670 MW

Specificity

Natural and recombinant mouse BCAM

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Basal cell adhesion molecule

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Basal cell adhesion molecule

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.