Human BCAM/Cd239 ELISA Kit PicoKine®

Human BCAM/Cd239 ELISA Kit PicoKine® (96 Tests) . Quantitate Human BCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 181 205 206 200 11.41 5.7% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 3 5441 6068 5199 5671 5594 320.17 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 181 205 206 200 11.41 5.7% Sample 1 210 181 205 206 200 11.41 5.7% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 3 5441 6068 5199 5671 5594 320.17 5.7% Sample 3 5441 6068 5199 5671 5594 320.17 5.7%

Synonyms

Basal cell adhesion molecule; Auberger B antigen; B-CAM cell surface glycoprotein; F8/G253 antigen; Lutheran antigen; Lutheran blood group glycoprotein; CD239; BCAM; LU, MSK19

Gene Name

BCAM

Gene ID

4059

UniProt

P50895

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E32-A547

Tissue Specificity

Wide tissue distribution (highest in the pancreas and very low in brain) . Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Applications

ELISA

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1%

Sensitivity

<15 pg/mL

Function

Laminin alpha-5 receptor. May mediate intracellular signaling. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

67405 MW

Specificity

Natural and recombinant human BCAM

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Basal cell adhesion molecule

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Basal cell adhesion molecule

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.