Mouse SPINT1/HAI-1 ELISA Kit PicoKine®

Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Spint1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 199 180 196 188 190 7.39 3.8% Sample 2 707 699 666 710 695 17.5 2.5% Sample 3 1860 1696 2012 1755 1830 120 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 199 180 196 188 190 7.39 3.8% Sample 1 199 180 196 188 190 7.39 3.8% Sample 2 707 699 666 710 695 17.5 2.5% Sample 2 707 699 666 710 695 17.5 2.5% Sample 3 1860 1696 2012 1755 1830 120 6.5% Sample 3 1860 1696 2012 1755 1830 120 6.5%

Synonyms

Kunitz-type protease inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; HAI-1; Spint1; Hai1

Gene Name

Spint1

Gene ID

20732

UniProt

Q9R097

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L507

Applications

ELISA

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 199 707 1860 185 728 1735 Standard deviation 14.72 41 119.04 16.4%1 48.1%3 140.53 CV (%) 7.4% 5.8% 6.4% 8.6% 6.2% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 199 707 1860 185 728 1735 Standard deviation 14.72 41 119.04 16.4%1 48.1%3 140.53 CV (%) 7.4% 5.8% 6.4% 8.6% 6.2% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 199 707 1860 185 728 1735 Mean (pg/ml) 199 707 1860 185 728 1735 Standard deviation 14.72 41 119.04 16.4%1 48.1%3 140.53 Standard deviation 14.72 41 119.04 16.4%1 48.1%3 140.53 CV (%) 7.4% 5.8% 6.4% 8.6% 6.2% 8.1% CV (%) 7.4% 5.8% 6.4% 8.6% 6.2% 8.1%

Sensitivity

<12 pg/mL

Function

Inhibitor of HGF activator. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

56591 MW

Specificity

Natural and recombinant mouse SPINT1

Anticoagulant

Heparin

Gene Name Synonym

Kunitz-type protease inhibitor 1

Subcellular Location

Secreted .

Protein Name

Kunitz-type protease inhibitor 1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.098 0.135 0.198 0.354 0.656 1.106 2.093 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.098 0.135 0.198 0.354 0.656 1.106 2.093 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.098 0.135 0.198 0.354 0.656 1.106 2.093 O.D. 0.034 0.098 0.135 0.198 0.354 0.656 1.106 2.093

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.