Human SPINT1/HAI-1 ELISA Kit PicoKine®

Human SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Sample 3 3990 4390 4302 4668 4337 241.86 5.5%

Synonyms

Kunitz-type protease inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; HAI-1; SPINT1; HAI1; UNQ223/PRO256

Gene Name

SPINT1

Gene ID

6692

UniProt

O43278

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G36-L529

Applications

ELISA

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8%

Sensitivity

<15 pg/mL

Function

Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

58398 MW

Specificity

Natural and recombinant human SPINT1

Anticoagulant

Heparin

Gene Name Synonym

Kunitz-type protease inhibitor 1

Subcellular Location

Secreted.

Protein Name

Kunitz-type protease inhibitor 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.