Human SPINT1/HAI-1 ELISA Kit PicoKine®

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Sample 3 3990 4390 4302 4668 4337 241.86 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.