Human LDLR ELISA Kit PicoKine®

The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 98 114 103 103 6.34 6.1% Sample 2 718 709 713 678 704 15.62 2.2% Sample 3 1395 1493 1364 1372 1406 51.5 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 98 114 103 103 6.34 6.1% Sample 1 99 98 114 103 103 6.34 6.1% Sample 2 718 709 713 678 704 15.62 2.2% Sample 2 718 709 713 678 704 15.62 2.2% Sample 3 1395 1493 1364 1372 1406 51.5 3.6% Sample 3 1395 1493 1364 1372 1406 51.5 3.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 718 1399 99 692 1264 Standard deviation 6.7%4 39.49 93.46 6.43 39.44 97.32 CV (%) 6% 5.5% 6.7% 6.5% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 718 1399 99 692 1264 Standard deviation 6.7%4 39.49 93.46 6.43 39.44 97.32 CV (%) 6% 5.5% 6.7% 6.5% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 718 1399 99 692 1264 Mean (pg/ml) 99 718 1399 99 692 1264 Standard deviation 6.7%4 39.49 93.46 6.43 39.44 97.32 Standard deviation 6.7%4 39.49 93.46 6.43 39.44 97.32 CV (%) 6% 5.5% 6.7% 6.5% 5.7% 7.7% CV (%) 6% 5.5% 6.7% 6.5% 5.7% 7.7%

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. Golgi apparatus. Early endosome. Late endosome. Cell surface. Lysosome. Found distributed from the plasma membrane to intracellular compartments. Localizes to the Golgi apparatus, early and late endosomes/lysosomes and cell surface in the presence of PCSK9.

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.051 0.1 0.183 0.327 0.599 1.131 1.865 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.051 0.1 0.183 0.327 0.599 1.131 1.865 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.051 0.1 0.183 0.327 0.599 1.131 1.865 O.D. 0.009 0.051 0.1 0.183 0.327 0.599 1.131 1.865

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.