Human Tryptase/TPSAB1, B2 ELISA Kit PicoKine®

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 276 255 284 276 272 10.75 3.9% Sample 2 1887 1991 1959 1839 1919 59.59 3.1% Sample 3 5608 5276 5559 5824 5566 195.24 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 276 255 284 276 272 10.75 3.9% Sample 1 276 255 284 276 272 10.75 3.9% Sample 2 1887 1991 1959 1839 1919 59.59 3.1% Sample 2 1887 1991 1959 1839 1919 59.59 3.1% Sample 3 5608 5276 5559 5824 5566 195.24 3.5% Sample 3 5608 5276 5559 5824 5566 195.24 3.5%

TPSAB1: Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level) .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7% 7.6% 7.5% 8.3% 9.3% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7% 7.6% 7.5% 8.3% 9.3% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7% 7.6% 7.5% 8.3% 9.3% 8.9% CV (%) 6.7% 7.6% 7.5% 8.3% 9.3% 8.9%

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.172 0.232 0.368 0.62 1.013 1.576 2.125 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.172 0.232 0.368 0.62 1.013 1.576 2.125 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.172 0.232 0.368 0.62 1.013 1.576 2.125 O.D. 0.067 0.172 0.232 0.368 0.62 1.013 1.576 2.125

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.