Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 8186 7996 8002 7330 7878 325.75 4.1% Sample 2 19826 19748 20656 19657 19971 399.55 2% Sample 3 93780 86259 87325 86965 88582 3025.32 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 8186 7996 8002 7330 7878 325.75 4.1% Sample 1 8186 7996 8002 7330 7878 325.75 4.1% Sample 2 19826 19748 20656 19657 19971 399.55 2% Sample 2 19826 19748 20656 19657 19971 399.55 2% Sample 3 93780 86259 87325 86965 88582 3025.32 3.4% Sample 3 93780 86259 87325 86965 88582 3025.32 3.4%

Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I; Apolipoprotein A-I (1-242) ; APOA1

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. .

Cardiovascular, Cholesterol Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8186 19826 93780 8183 19180 85400 Standard deviation 376.55 1447.29 4876.56 507.34 1514.62 5209.4 CV (%) 4.6% 7.3% 5.2% 6.2% 7.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8186 19826 93780 8183 19180 85400 Standard deviation 376.55 1447.29 4876.56 507.34 1514.62 5209.4 CV (%) 4.6% 7.3% 5.2% 6.2% 7.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 8186 19826 93780 8183 19180 85400 Mean (pg/ml) 8186 19826 93780 8183 19180 85400 Standard deviation 376.55 1447.29 4876.56 507.34 1514.62 5209.4 Standard deviation 376.55 1447.29 4876.56 507.34 1514.62 5209.4 CV (%) 4.6% 7.3% 5.2% 6.2% 7.9% 6.1% CV (%) 4.6% 7.3% 5.2% 6.2% 7.9% 6.1%

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT) . As part of the SPAP complex, activates spermatozoa motility. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.008 0.042 0.079 0.17 0.366 0.826 1.693 2.485 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.008 0.042 0.079 0.17 0.366 0.826 1.693 2.485 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.008 0.042 0.079 0.17 0.366 0.826 1.693 2.485 O.D. 0.008 0.042 0.079 0.17 0.366 0.826 1.693 2.485

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.