Human APOE/Apolipoprotein E ELISA Kit PicoKine®

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7334 7205 6656 6991 7046 256.58 3.6% Sample 2 30860 28598 30251 27221 29232 1426.08 4.8% Sample 3 101114 104676 102789 88986 99391 6138.2 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7334 7205 6656 6991 7046 256.58 3.6% Sample 1 7334 7205 6656 6991 7046 256.58 3.6% Sample 2 30860 28598 30251 27221 29232 1426.08 4.8% Sample 2 30860 28598 30251 27221 29232 1426.08 4.8% Sample 3 101114 104676 102789 88986 99391 6138.2 6.1% Sample 3 101114 104676 102789 88986 99391 6138.2 6.1%

Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL) . APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7334 30860 101114 7480 33927 97046 Standard deviation 506.04 2191.06 7886.89 598.4 2917.72 7957.77 CV (%) 6.9% 7.1% 7.8% 8% 8.6% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7334 30860 101114 7480 33927 97046 Standard deviation 506.04 2191.06 7886.89 598.4 2917.72 7957.77 CV (%) 6.9% 7.1% 7.8% 8% 8.6% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 7334 30860 101114 7480 33927 97046 Mean (pg/ml) 7334 30860 101114 7480 33927 97046 Standard deviation 506.04 2191.06 7886.89 598.4 2917.72 7957.77 Standard deviation 506.04 2191.06 7886.89 598.4 2917.72 7957.77 CV (%) 6.9% 7.1% 7.8% 8% 8.6% 8.2% CV (%) 6.9% 7.1% 7.8% 8% 8.6% 8.2%

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.055 0.199 0.291 0.443 0.711 1.156 1.676 2.315 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.055 0.199 0.291 0.443 0.711 1.156 1.676 2.315 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.055 0.199 0.291 0.443 0.711 1.156 1.676 2.315 O.D. 0.055 0.199 0.291 0.443 0.711 1.156 1.676 2.315

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.