Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 76 69 64 70 69 4.26 6.1% Sample 2 257 230 240 246 243 9.78 4% Sample 3 1026 878 895 954 938 57.98 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 76 69 64 70 69 4.26 6.1% Sample 1 76 69 64 70 69 4.26 6.1% Sample 2 257 230 240 246 243 9.78 4% Sample 2 257 230 240 246 243 9.78 4% Sample 3 1026 878 895 954 938 57.98 6.1% Sample 3 1026 878 895 954 938 57.98 6.1%

Cancer, Cancer Metabolism, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 257 1026 81 240 1095 Standard deviation 4.63 12.33 41.04 4.01 11.52 53.65 CV (%) 6.1% 4.8% 4% 7.3% 4.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 257 1026 81 240 1095 Standard deviation 4.63 12.33 41.04 4.01 11.52 53.65 CV (%) 6.1% 4.8% 4% 7.3% 4.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 76 257 1026 81 240 1095 Mean (pg/ml) 76 257 1026 81 240 1095 Standard deviation 4.63 12.33 41.04 4.01 11.52 53.65 Standard deviation 4.63 12.33 41.04 4.01 11.52 53.65 CV (%) 6.1% 4.8% 4% 7.3% 4.8% 4.9% CV (%) 6.1% 4.8% 4% 7.3% 4.8% 4.9%

Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes inly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen . Cytoplasm . Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.175 0.27 0.411 0.665 1.043 1.608 2.451 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.175 0.27 0.411 0.665 1.043 1.608 2.451 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.175 0.27 0.411 0.665 1.043 1.608 2.451 O.D. 0.035 0.175 0.27 0.411 0.665 1.043 1.608 2.451

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.