Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 82 82 97 89 7.5 8.4% Sample 2 301 311 299 294 301 6.17 2% Sample 3 903 978 956 925 940 28.69 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 82 82 97 89 7.5 8.4% Sample 1 97 82 82 97 89 7.5 8.4% Sample 2 301 311 299 294 301 6.17 2% Sample 2 301 311 299 294 301 6.17 2% Sample 3 903 978 956 925 940 28.69 3% Sample 3 903 978 956 925 940 28.69 3%

Signal Transduction, Immunology, Microbiology, Cancer, Signaling Pathway, Cytoskeleton/ECM, Calcium Signaling, Extracellular Matrix, Invasion/Microenvironment, ECM Proteins, Innate Immunity, Macrophage/Inflammation, Defensin, Human Protein, Protein, NK Cells, Complement, ECM Enzymes

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 301 903 95 322 978 Standard deviation 7.66 17.75 69.53 7.69 19.32 76.28 CV (%) 7.9% 5.3% 7.7% 8.1% 6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 301 903 95 322 978 Standard deviation 7.66 17.75 69.53 7.69 19.32 76.28 CV (%) 7.9% 5.3% 7.7% 8.1% 6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 301 903 95 322 978 Mean (pg/ml) 97 301 903 95 322 978 Standard deviation 7.66 17.75 69.53 7.69 19.32 76.28 Standard deviation 7.66 17.75 69.53 7.69 19.32 76.28 CV (%) 7.9% 5.3% 7.7% 8.1% 6% 7.8% CV (%) 7.9% 5.3% 7.7% 8.1% 6% 7.8%

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.121 0.199 0.345 0.567 1.032 1.801 2.929 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.121 0.199 0.345 0.567 1.032 1.801 2.929 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.121 0.199 0.345 0.567 1.032 1.801 2.929 O.D. 0.031 0.121 0.199 0.345 0.567 1.032 1.801 2.929

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.