GFP/Green fluorescent protein ELISA Kit PicoKine®

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 34 38 35 36 2.06 5.7% Sample 2 220 216 221 238 223 8.43 3.7% Sample 3 444 430 423 422 429 8.78 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 34 38 35 36 2.06 5.7% Sample 1 39 34 38 35 36 2.06 5.7% Sample 2 220 216 221 238 223 8.43 3.7% Sample 2 220 216 221 238 223 8.43 3.7% Sample 3 444 430 423 422 429 8.78 2% Sample 3 444 430 423 422 429 8.78 2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 444 38 224 429 Standard deviation 1.98 17.16 28.86 2.16 20.16 30.88 CV (%) 7.2% 7.8% 6.5% 5.7% 9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 444 38 224 429 Standard deviation 1.98 17.16 28.86 2.16 20.16 30.88 CV (%) 7.2% 7.8% 6.5% 5.7% 9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 444 38 224 429 Mean (pg/ml) 39 220 444 38 224 429 Standard deviation 1.98 17.16 28.86 2.16 20.16 30.88 Standard deviation 1.98 17.16 28.86 2.16 20.16 30.88 CV (%) 7.2% 7.8% 6.5% 5.7% 9% 7.2% CV (%) 7.2% 7.8% 6.5% 5.7% 9% 7.2%

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca (2+) -activated photoprotein aequorin.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.085 0.138 0.231 0.417 0.724 1.268 2.184 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.085 0.138 0.231 0.417 0.724 1.268 2.184 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.085 0.138 0.231 0.417 0.724 1.268 2.184 O.D. 0.012 0.085 0.138 0.231 0.417 0.724 1.268 2.184

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.