Rat BMP-7 ELISA Kit PicoKine®

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 94 87 84 87 3.76 4.3% Sample 2 190 232 197 213 208 16.17 7.7% Sample 3 752 848 801 765 791 37.23 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 94 87 84 87 3.76 4.3% Sample 1 86 94 87 84 87 3.76 4.3% Sample 2 190 232 197 213 208 16.17 7.7% Sample 2 190 232 197 213 208 16.17 7.7% Sample 3 752 848 801 765 791 37.23 4.7% Sample 3 752 848 801 765 791 37.23 4.7%

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Organogenesis, Pathways and Processes, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.