Rat Eotaxin ELISA Kit PicoKine®

Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 51 51 53 52 1.00 1.9% Sample 2 222 212 240 236 227 11.16 4.9% Sample 3 1050 1017 957 1091 1028 49.02 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 51 51 53 52 1.00 1.9% Sample 1 53 51 51 53 52 1.00 1.9% Sample 2 222 212 240 236 227 11.16 4.9% Sample 2 222 212 240 236 227 11.16 4.9% Sample 3 1050 1017 957 1091 1028 49.02 4.7% Sample 3 1050 1017 957 1091 1028 49.02 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 222 1050 58 464 1127 Standard deviation 3.71 12.88 64.05 3.07 19.49 66.50 CV (%) 7% 5.8% 6.1% 5.3% 4.2% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 222 1050 58 464 1127 Standard deviation 3.71 12.88 64.05 3.07 19.49 66.50 CV (%) 7% 5.8% 6.1% 5.3% 4.2% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 222 1050 58 464 1127 Mean (pg/ml) 53 222 1050 58 464 1127 Standard deviation 3.71 12.88 64.05 3.07 19.49 66.50 Standard deviation 3.71 12.88 64.05 3.07 19.49 66.50 CV (%) 7% 5.8% 6.1% 5.3% 4.2% 5.9% CV (%) 7% 5.8% 6.1% 5.3% 4.2% 5.9%

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.085 0.141 0.258 0.449 0.924 1.634 2.885 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.085 0.141 0.258 0.449 0.924 1.634 2.885 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.085 0.141 0.258 0.449 0.924 1.634 2.885 O.D. 0.023 0.085 0.141 0.258 0.449 0.924 1.634 2.885

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.