Human CCL28 ELISA Kit PicoKine®

CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 82 79 69 75 5.06 6.7% Sample 2 277 266 257 276 269 8.15 3% Sample 3 866 893 781 873 853 42.87 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 82 79 69 75 5.06 6.7% Sample 1 73 82 79 69 75 5.06 6.7% Sample 2 277 266 257 276 269 8.15 3% Sample 2 277 266 257 276 269 8.15 3% Sample 3 866 893 781 873 853 42.87 5% Sample 3 866 893 781 873 853 42.87 5%

Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 277 866 77 254 867 Standard deviation 5.03 21.32 59.75 5.77 20.32 64.15 CV (%) 6.9% 7.7% 6.9% 7.5% 8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 277 866 77 254 867 Standard deviation 5.03 21.32 59.75 5.77 20.32 64.15 CV (%) 6.9% 7.7% 6.9% 7.5% 8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 277 866 77 254 867 Mean (pg/ml) 73 277 866 77 254 867 Standard deviation 5.03 21.32 59.75 5.77 20.32 64.15 Standard deviation 5.03 21.32 59.75 5.77 20.32 64.15 CV (%) 6.9% 7.7% 6.9% 7.5% 8% 7.4% CV (%) 6.9% 7.7% 6.9% 7.5% 8% 7.4%

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.168 0.238 0.365 0.606 1.032 1.648 2.68 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.168 0.238 0.365 0.606 1.032 1.648 2.68 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.168 0.238 0.365 0.606 1.032 1.648 2.68 O.D. 0.056 0.168 0.238 0.365 0.606 1.032 1.648 2.68

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.